Vacutainer or Blood collection Tube , Part : 1

Blood Collection tubes

Part : 1

Blood Collection Tube

This study material on blood collection tube which is topic of phlebotomy. There are many tubes are used in medical laboratory which contains different colours top, indication and purpose of this different colour of tube we will study in this topic.

Pink Top Tube

Pink Top Tube

Anti Coagulant - EDTA (Ethylene Diamine Tetra Acetic Acid)

Mode of Action - Formation of Calcium Salt and removal of calcium.

Use -

- Specially used for whole blood sample being sent to transfusion Lab. - Cross match
- Blood grouping
- Direct coomb's test.

Note : Mix well with EDTA by inverting tube atleast 8 times.

Gray Top Tube 

Gray Top Tube

Anti Coagulant - Potassium Oxalate

Other agent Contain - Sodium Fluoride

Mode of Action - Sodium fluoride acts as the glycolytic inhibitor and prevents the cell in the blood from utilizing the glucose.

Use :-

 - FBS test
 - GTT test

 - Lactate test

Black Top Tube 

Black Top Tube

Anti Coagulant - Sodium Citrate

Mode of Action -
Formation of Calcium salt an removal of calcium.

Use - ESR (Erythrocyte Sedimentation Rate) test

Ratio of sample and Anti Coagulant is 1:4 in this black top tube.

Red Top Tube 

Red Top Tube 

It is clot activator tube.

Anti coagulant - No ( Absent)

Mode of Action -
Blood clots and serum separated by centrifugation.

Use -
Chemistry,blood bank (cross match,Immunology,serology.

Note : Do not centrifuge soon after sample collection that means centrifuge after atleast 30 minutes of sample collection.