Vacutainer or Blood collection Tubes , Part : 3

Blood Collection Tubes

Part : 3

Vacutainer or Blood collection tubes

Hi friends we will study some more blood collection tubes in this topic.

This topic is very interesting for you so refer whole this topic.so let's start this topic.

Gold Top Tube 

Gold Top Tube or SST tubes

- It is also known as Serum separating tube (SST) 

Anti coagulant - No ( Absent)

Contain two agents - 

1) Silica Particle : It is activated clotting and cause blood cells to clump together.

2) Serum separator : Inert gel which is separate serum and blood cells.

Mode of Action - Serum separated by gel.

Use :- 

Endocrinology,oncology,toxicology, microbiology, immunology

Some tests done by this tube are : 

Urea , Tumour markers

Bone profile (Calcium, Po4 , ALP)

CRP , Immunoglobulin

LFT , Electrolytes ( Na,K,Ca,Iron)

Albumin , Troponin , RA factor, etc.

Light Yellow Top Tube

Light yellow top tube 

Anti coagulant - ACD (Acid Citrate Dextrose)

Mode of action - Complement Inactivation.

Use :-

- Blood bank
- Blood culture
- HLA Phenotypic
- Paternity testing

Purple or Lavender Top Tube

Purple or Lavender top tube

Anti coagulant - EDTA

Mode of action - Formation of calcium salt and removal of calcium.

Use :-

Hematology, Blood bank,Whole blood , Molecular genetics.

Tests done by this tube are :

CBC , PTH, HBA1C , ESR , Reticulocyte
Blood smear, Red cell folate,
Electrophoresis,etc...

Note : Mix well with EDTA.

White Top Tube 

White top tube 

Anti coagulant - EDTA with gel.

Use :- Used for molecular diagnosis 

 Like, PCR, DNA amplification

Orange Top Tube

Orange top tube 

It is a thrombin based activator

Anti coagulant - Throbin

Use :- Used for stat serum testing.

● Now we will see difference and similarity between Light Blue Top Tube and Black Top Tube.

Light Blue Top Tube

Anti coagulant - Sodium Citrate

Dilution Ratio - 1 : 9

Sodium citrate - 3.2 %

Special test - ESR not done by this tube

Black Top Tube 

Anti coagulant - Sodium Citrate

Dilution Ratio - 1 : 4

Sodium citrate - 3.8 %

Special test - ESR done by this tube.